Overlay Options – Population Color.Īs you add a population to an overlay in the Layout Editor, FlowJo will assign a color to the population as well as a name in the Legend. For more information on changing displays within the Layout Editor, go here. We will discuss the options most pertinent to overlays below. You can change the order of populations within an overlay, scaling (histograms), axes, axes labels, fonts, colors, add presets, etc. Overlays, as well as any other type of plot in the Layout Editor, can be modified in multiple ways. You can drag populations in from the Workspace, or from the Layout Editor.Īs you add populations to the Overlay, FlowJo keeps track of them in the Legend and assigns a different color to each additional population. When an overlay is allowed, you’ll see the borders of the chart box of the first population highlighted (in blue) to signify that it will accept the contents of the drag. The resulting Overlay type is dependent on the plot being dragged into (i.e if you started with a histogram and dragged in a dot plot, you will get a histogram overlay). Please note, you can drag and drop a histogram onto a dot plot or vise versa. To add additional populations to the Overlay, simply repeat the process, or select multiple populations from the Workspace and drag-and-drop them on a plot in the Layout Editor at once. a dot plot if you were looking at scatter or fluorescent parameters on both the X- and Y-axes). FlowJo will create an Overlay based on the type of plot that was last used in the Graph Window for that population (e.g. Then drag a second population on top of the first one. Creating an Overlay in the Layout Editor.Ĭreating Overlays in FlowJo v10 is fast and easy. To create an overlay, drag a population from the Workspace into the Layout Editor. Histogram overlays have the added benefit of being displayed offset to one another, the controls of which are discussed further below. Find rare populations, identify various subsets, define cytokine expressing cells, compare controls to experimentals and many other functions with overlays.įlowJo vX allows you to create both univariate ( histograms, cumulative distribution function) and bivariate ( dot, pseudocolor, density, contour, and zebra plots) Overlays within the Layout Editor. Differences between populations within a single experiment or amongst groups of experiments are easily seen. Overlaying univariate or bivariate plots is a powerful visual tool to for cytometric analyses. Please navigate through the pages below to dive into more specific details of the Table Editor.Compare a control to an experimental set, evaluate the accuracy of gates, find high or low expressors, or a perform a myriad of other analyses using Overlays in FlowJo v10. When the table is created, it will batch automatically for all samples in that group.Įach column in FlowJo’s tables can be independently adjusted to include special formatting options ( heat mapping), setting a control value ( won’t iterate), or hidden (calculate but don’t show – useful for equations of equations when you don’t want to show the primary equation). *Please note: when you batch your statistics to the group in the workspace, you only need to drag prototypic statistics from the FIRST sample into the Table Editor. They can be generated for viewing within FlowJo, or exported to many formats and manipulated outside of FlowJo.įlowJo’s tables can be batched, similarly to layouts, including the use of iteration. Tables can be used to show summary statistics, sample keywords, or custom formulas. The Table Editor is FlowJo’s platform for exporting your analyses’ numerical attributes.

Flowjo table editor